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KMID : 0364820090450010069
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Korean Journal of Microbiology
2009 Volume.45 No. 1 p.69 ~ p.73
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Production Condition and Characterization of Extracellular Protease from Micrococcus
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Cha In-Tae
Roh Dong-Hyun
Oh Yong-Sik
Cho Woon-Dong
Lim Chae-Sung
Lee Je-Kwan
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Abstract
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Proteases are degradative enzymes which hydrolyze a peptide bond between amino acids and they are abundantly
applied to commercial field. In order to investigate optimal medium compositions of carbon and nitrogen source for enzyme production, modified STY medium containing 0.15% yeast extract were used as basal medium. When galactose was used as carbon source, enzyme activity showed 1.3 higher than that of glucose. For nitrogen source addition of casamino acids to basal medium in place of tryptone showed lowest activity, whereas addition of malt extract showed maximal activity. The optimum temperature and pH of extracellular protease were found to be 35oC an pH 8.5.
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KEYWORD
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extracellular protease, medium compositions, Micrococcus sp. HJ19, optimal temperature & pH
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